Title:
CAS VARIANTS FOR GENE EDITING
Document Type and Number:
Japanese Patent JP2022043042
Kind Code:
A
Abstract:
To provide strategies, systems, reagents, methods and kits that are useful for targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome.SOLUTION: In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or nucleic acid editing enzyme domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or nucleic acid editing domains, are provided.SELECTED DRAWING: Figure 3
Inventors:
DAVID R LIU
ALEXIS CHRISTINE KOMOR
ALEXIS CHRISTINE KOMOR
Application Number:
JP2021188589A
Publication Date:
March 15, 2022
Filing Date:
November 19, 2021
Export Citation:
Assignee:
HARVARD COLLEGE
International Classes:
C12N15/09; C12N9/14; C12N15/55; C12N15/62; C12Q1/686; C12Q1/6897
Domestic Patent References:
| JP2017500035A | 2017-01-05 |
Foreign References:
| WO2013176772A1 | 2013-11-28 | |||
| US20110104787A1 | 2011-05-05 | |||
| WO2010132092A2 | 2010-11-18 |
Other References:
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3 BIOTECH., vol. 3, JPN6019005546, 18 September 2012 (2012-09-18), pages 225 - 234, ISSN: 0004928084
BRITISH J. HAEMATOL., vol. 82, JPN6019005552, 1992, pages 66 - 72, ISSN: 0004928085
GENOMICS, vol. 17, JPN6022049776, 1993, pages 740 - 743, ISSN: 0004928086
SCIENCE, vol. 333, JPN6015052349, 2011, pages 1843 - 1846, ISSN: 0004928087
PROTEIN SCI., vol. 13, JPN6019005553, 2004, pages 694 - 702, ISSN: 0004928088
J. ALZHEIMER'S DISEASE, vol. 25, JPN6019005551, 2011, pages 425 - 431, ISSN: 0004928089
Attorney, Agent or Firm:
Kiyokazu