Title:
CAS9 VARIANTS AND METHODS OF USE
Document Type and Number:
Japanese Patent JP2023011727
Kind Code:
A
Abstract:
To provide a more effective, efficient, or otherwise more robust or flexible Cas-based genome modification methods for modifying/altering a genomic target site in a prokaryotic or eukaryotic cell.SOLUTION: Compositions and methods are provided for variant Cas systems and elements comprising such systems, for example, Cas endonuclease variants, guide polynucleotide/Cas endonuclease complexes comprising Cas endonuclease variants, as well as guide polynucleotides and guide RNA elements that can interact with Cas endonuclease variants. Compositions and methods are provided for genome modification of a target site in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system comprising a Cas9 endonuclease variant to provide an effective system for modifying or altering target sequences within the genome of a cell or organism.SELECTED DRAWING: Figure 1
Inventors:
Frisch, Ryan El
Hu, Hong Shan
Hu, Hong Shan
Application Number:
JP2022171188A
Publication Date:
January 24, 2023
Filing Date:
October 26, 2022
Export Citation:
Assignee:
Danisco US Inc.
International Classes:
C12N15/55; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12N15/09
Attorney, Agent or Firm:
Yamazaki Yuzo
Toshiaki Akamatsu
Tadao Naito
Chihiro Imai
Park Shinon
Toshiaki Akamatsu
Tadao Naito
Chihiro Imai
Park Shinon
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