To provide a cell adsorbent that strongly attaches or adsorbs various cells, a cell culture substrate, a method for producing the same, a cell passage method, and a cell proliferation method.
The cell culture substrate includes a cell adsorbent composed of at least one kind selected from animal-derived fibrous or filmy natural organic polymers chemically modified and processed or graft processed. The cell culture substrate is produced by immersing the natural organic polymer in a graft processing liquid comprising a surfactant aqueous solution and a monomer containing fluorine, an aqueous solution containing a phosphoric monoester, methacrylamide and 2-hydroxyethyl methacrylate or an organic solvent solution of a dibasic acid anhydride or epoxy compound and performing graft processing or chemical modification processing in the presence of a polymerization initiator. Cells are passaged using the cell culture substrate in an undifferentiated state in a proliferative environment and the cells are proliferated in a proliferative environment.
ABE KOJI
OZAWA YOKO
SASAKI KATSUNORI
| JPH10127752A | 1998-05-19 | |||
| JP2009148276A | 2009-07-09 | |||
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| JP2006158305A | 2006-06-22 | |||
| JP2005501662A | 2005-01-20 | |||
| JPH1152303A | 1999-02-26 | |||
| JP2003011312A | 2003-01-15 |
| WO2007074747A1 | 2007-07-05 |
Muranaka