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Title:
CELL CULTURE CONTAINER, ITS PRODUCTION AND CULTURE
Document Type and Number:
Japanese Patent JP3270286
Kind Code:
B2
Abstract:

PURPOSE: To provide a cell culture container for forming a cytoagglutinin useful for judging cytotoxicity of a chemical and a chemical substance, capable of producing a large amount of each a cytoagglutinin at a stretch, by providing the bottoms of wells of each a specific shape with a hydrophilic film comprising a mixed composition composed of a styrenic polymer and a phospholipid.
CONSTITUTION: The bottoms of plural holes (wells) to be zones for culturing cells have a funnel shape with ≤120 degrees, a hemispherical shape having ≤10mm curvature radius or a shape prepared by flattening the central part of these bottom faces and at least the bottom face of the inside of each well is provided with a hydrophilic film comprising a mixed composition composed of a styrenic polymer and preferably 1-30wt.% of a phospholipid such as phosphatidylcholine to give the objective cell culture container for forming a cytoagglutinin.


Inventors:
Kenji Kawamura
Hideaki Asai
Hayao Tanaka
Application Number:
JP5427295A
Publication Date:
April 02, 2002
Filing Date:
March 14, 1995
Export Citation:
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Assignee:
Sumitomo Bakelite Co., Ltd.
International Classes:
C12M1/20; C12M1/22; C12M1/34; C12M3/00; C12N5/07; C12N5/071; (IPC1-7): C12M3/00; C12M1/20; C12M1/22; C12M1/34
Domestic Patent References:
JP646831A
JP249898U
JP63129500U