PURPOSE: To efficiently obtain polyploidy cells useful, e.g. for producing a new- breed plant by carrying out cell fusion between a protoplast of an Eucalyptus plant and a transformat protoplast, subsequently culturing it in the presence of a herbaceous plant protoplast and then culturing it in an antibiotic-containing medium.
CONSTITUTION: To a callus obtained from a shoot anlage of an Eucalyptus plant (e.g. Eucalyptus saligna) and a callus obtained from a shoot anlage of a transformat Eucalyptus plant, an enzyme solution containing cellulase, pectolyase, etc., is added and the resultant mixture is treated at 27°C for 16hr to isolate the respective protoplasts of two kinds of Eucalyptus plants for cell fusion. The resultant isolated protoplasts are then subjected to cell fusion by using a 40wt.% polyethylene glycol solution, etc., and the fused cell is subsequently cultured in the presence of a protoplast of a herbaceous plant (e.g. Hibiscu cannabinus) excellent in fissiparity to obtain a colony. The obtained colony is cultured in an antibiotic-containing culture medium, thus efficiently selecting the objective polyploid-candidate colony.
DOI KEIGO
ITO KAZUYA
