Title:
細胞溶解プロセス
Document Type and Number:
Japanese Patent JP2014507157
Kind Code:
A
Abstract:
A process for cell lysis is provided. The process comprises passing a mixture comprising a suspension of cells and a lysis reagent through a flow-through reactor, wherein the mixture passes through the reactor with pulsed or superimposed oscillating flow. The process is preferably employed for the preparation of biological products, such as pDNA or inclusion bodies.
Inventors:
John McDonald, Liddell
Razak, Asam
Razak, Asam
Application Number:
JP2013554935A
Publication Date:
March 27, 2014
Filing Date:
February 17, 2012
Export Citation:
Assignee:
Fujifilm Diosynth Biotechnologies-UK Limited
International Classes:
C12N1/06; C12P19/34; C12P21/00; C12M1/33; C12M1/42
Domestic Patent References:
| JP2008527312A | 2008-07-24 |
Foreign References:
| WO2006060282A2 | 2006-06-08 | |||
| EP0191941A1 | 1986-08-27 |
Other References:
JPN6015053092; Ｓａｅｔｈａｗｔ Ｃｈａｍｓａｒｔ ｅｔ ａｌ．: 'Ａｌｋａｌｉｎｅ-Ｃｅｌｌ Ｌｙｓｉｓ Ｔｈｒｏｕｇｈ Ｉｎ-Ｌｉｎｅ ＳｔａｔｉｃＭｉｘｅｒ Ｒｅａｃｔｏｒ ｆｏｒ ｔｈｅ Ｐｒｏｄｕｃｔｉｏｎ ｏｆ Ｐｌａｓｍｉｄ ＤＮＡ ｆｏｒ Ｇｅｎｅ Ｔ' Ｂｉｏｔｅｃｈｎｏｌｏｇｙ Ｂｉｏｅｎｇｉｎｅｅｒｉｎｇ Ｖｏｌ．９６， Ｎｏ．３, 2007, ｐ．４７１-４８２
Attorney, Agent or Firm:
Shinjiro Ono
Yasushi Kobayashi
Shigeo Takeuchi
Osamu Yamamoto
Noriko Tsujimoto
Yasushi Kobayashi
Shigeo Takeuchi
Osamu Yamamoto
Noriko Tsujimoto