Title:
細胞分離装置及び細胞分離方法
Document Type and Number:
Japanese Patent JP6762088
Kind Code:
B2
Abstract:
The present invention provides a cell isolation device and cell isolation method capable of selecting cells having high antibody-production ability, from a candidate cell group by using a simple process but causing less damage to the cells. The cell isolation device (100) is provided with an operation part (101) in which a culture vessel having a plurality of wells is placed, an inoculation device (103) for inoculating candidate cells into a culture medium, a temperature adjustment device (102) for adjusting the temperature of the culture medium, a reagent addition device (106) for adding an anti-membrane protein antibody to the culture medium, and a collection device (104) for collecting the culture medium. A protein-binding site having an antibody-binding ability, and a temperature responsive site of which the three-dimensional structure changes depending on a change in temperature, are bound to the inner wall surface of the wells. The anti-membrane protein antibody is added to the cultured candidate cells, and the temperature of the culture medium is changed to a temperature exceeding the phase transition temperature of the temperature responsive site, whereby only antibody-producing cells are isolated and collected.
Inventors:
Keisuke Shibuya
Maruyama Yuji
Hirai Chizuru
Maruyama Yuji
Hirai Chizuru
Application Number:
JP2015208259A
Publication Date:
September 30, 2020
Filing Date:
October 22, 2015
Export Citation:
Assignee:
株式会社日立製作所
International Classes:
C12M1/00; B01D15/00; B01J20/26; B01J20/34; C12M3/00; C12N1/02; C12N5/0781
Domestic Patent References:
| JP2006223195A | ||||
| JP2016163550A | ||||
| JP2016165677A |
Foreign References:
| WO2015033457A1 |
Attorney, Agent or Firm:
Isono International Patent and Trademark Office