Title:
細胞シグナル応答型遺伝子転写制御系
Document Type and Number:
Japanese Patent JP4290352
Kind Code:
B2
Abstract:
A gene complex-forming material which comprises a water-soluble polymer having a peptide containing an amino acid sequence serving as the substrate of an intracellular signal-responsive enzyme and basic amino acids imparting cationic nature; a gene complex composed of this gene complex-forming material with a gene; and a gene transfer method and a gene transfer agent with the use of the same. Namely, a novel material and a method wherein the cationic moiety of the peptide and the gene form a rigid ion complex to give a stable gene complex, and, upon a cellular signal response, the positive charge of the cationic moiety of the peptide is neutralized or disappears and the gene complex is broken in the cell to thereby release the gene, thus activating the gene transferred into specific cells. The neutralization or disappearance of the positive charge can be achieved by, for example, phosphorylation with protein kinase A or cleavage by caspase.
Inventors:
Yoshiki Katayama
Application Number:
JP2001202064A
Publication Date:
July 01, 2009
Filing Date:
July 03, 2001
Export Citation:
Assignee:
Japan Science and Technology Agency
International Classes:
C12N15/09; A61K47/42; A61K47/48; A61K48/00; A61P43/00; C07K7/06; C07K14/47; C07K19/00; C12N15/87
Other References:
榊原茂基 他,細胞内カスパーゼシグナル応答性遺伝子制御システム,高分子学会予稿集,2001年 5月 7日,第50巻,p.975 IIPd136
片山佳樹 他,細胞内リン酸化シグナル応答性遺伝子制御システム,高分子学会予稿集,2001年 5月 7日,第50巻,p.975 IIPc135
片山佳樹,細胞情報と化学情報を相互変換する分子の創製と機能,日本化学会九州支部・同中国四国支部 支部合同鹿児島大会 講演要旨集,2000年10月28日,p.297-298
片山佳樹 他,細胞内リン酸化シグナル応答性遺伝子制御システム,高分子学会予稿集,2001年 5月 7日,第50巻,p.975 IIPc135
片山佳樹,細胞情報と化学情報を相互変換する分子の創製と機能,日本化学会九州支部・同中国四国支部 支部合同鹿児島大会 講演要旨集,2000年10月28日,p.297-298
Attorney, Agent or Firm:
Norio Saeki