Title:
セルラーゼ変異体
Document Type and Number:
Japanese Patent JP3532576
Kind Code:
B2
Abstract:
A method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:
a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG;
b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV;
c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5Å;
d. identifying surface-exposed amino acid residues of the enzyme;
e. identifying all charged or potentially charged amino acid residue positions of the enzyme;
f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided;
g. carrying out the substition, deletion or insertion by using conventional protein engineering techniques; and cellulase variants obtained by the method.
Inventors:
アンデルセン，キム ビルボール
クリスチャンセン，ラルス
シューレイン，マルティン
ダムガールト，ボー
フォン デル オステン，クラウス
クリスチャンセン，ラルス
シューレイン，マルティン
ダムガールト，ボー
フォン デル オステン，クラウス
Application Number:
JP51420098A
Publication Date:
May 31, 2004
Filing Date:
September 17, 1997
Export Citation:
Assignee:
ノボザイムス アクティーゼルスカブ
International Classes:
C12N15/09; C11D3/386; C12N1/15; C12N1/21; C12N9/42; C12S11/00; C12S99/00; D06L4/40; D06M16/00; D21B1/02; D21C5/02; D21H21/24; C12R1/645; (IPC1-7): C12N9/42; C12N15/09
Attorney, Agent or Firm:
石田 敬 （外８名）