Title:
セルロース分解酵素遺伝子及び該遺伝子の利用
Document Type and Number:
Japanese Patent JP4385766
Kind Code:
B2
Abstract:
Treating woodchips comprising: (a) production of DNA encoding a specific antisense RNA; (b) constructing a vector; (c) effecting transcription by using the vector and preparing host cells with suppressed cellulose-digesting enzyme activity; and (d) inoculating the woodchips with the host cells for treatment, is new. Treating woodchips comprises: (a) production of a DNA encoding an antisense RNA which is substantially complementary to the whole transcription product of a Basidomycetes-originated cellulose-digesting enzyme gene or a part of it; (b) constructing a vector which contains: (i) the already-specified DNA; or (ii) a recombinant DNA containing the DNA and a DNA fragment having promoter activity where the DNA is ligated to the DNA fragment so that the antisense RNA of the cellulose-digesting enzyme is formed by transcription of the DNA; (c) effecting transcription by using the vector and preparing host cells with suppressed cellulose-digesting enzyme activity; and (d) inoculating the woodchips with the host cells for treatment. Independent claims are also included for: (1) woodchips produced after treatment; (2) producing pulp by using the wood chips; and (3) pulp obtained by the process of (2).
Inventors:
Akira Tsukamoto
Seiji Nakagame
Instep truth
Jun Sugiura
Sakaguchi Satoko
Atsushi Kojo
Seiji Nakagame
Instep truth
Jun Sugiura
Sakaguchi Satoko
Atsushi Kojo
Application Number:
JP2003569833A
Publication Date:
December 16, 2009
Filing Date:
February 25, 2003
Export Citation:
Assignee:
Oji Paper Co.,Ltd.
International Classes:
D21C3/22; C12N9/04; C12N9/42; C12N15/09; D21C5/00; C12R1/645
Domestic Patent References:
| JP2000342275A | ||||
| JP2000106887A | ||||
| JP11127863A |
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Yumi Naito
Satoshi Fujita
Yumi Naito