Title:
CHIMERA ANTIBODY AGAINST HUMAN-INTERLEUKIN-6 RECEPTOR
Document Type and Number:
Japanese Patent JP3176598
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a novel chimera antibody against human interleukin-6 receptor that comprises a chimera L-chain having the L-chain C region of human antibody as well as the L-chain V-region of mouse monoclonal antibody against human interleukin-6 receptor, thus shows low immunogenicity and is useful as a therapeutic agent for myeloma.
SOLUTION: This chimera antibody has a novel chimera L-chain that has the L-chain C-region of human antibody and the L-chain V-region of mouse monoclonal antibody against human interleukin-6 (IL-6) receptor, where this L-chain V-region has amino acid sequences of formula I to formula III. When it is dosed to human, the chimera antibody shows low immunogenicity and is useful as a therapeutic agent or the like for myeloma. The chimera antibody is prepared by linking a cDNA clone encoding the L-chain and H-chain V-region of mouse monoclonal antibody against the human IL-6 receptor to a cDNA encoding the human C region connecting to the expression vector for mammalian cells to prepare a recombinant expression vector, transducing the vector into a mammalian cell, for example, COS cell or the like and expressing the vector.
Inventors:
Masayuki Tsuchiya
Isao Sato
Bendig, Mary Margaret
Stephen Tarren, Jones
Jose William, Saldana
Isao Sato
Bendig, Mary Margaret
Stephen Tarren, Jones
Jose William, Saldana
Application Number:
JP33828699A
Publication Date:
June 18, 2001
Filing Date:
April 24, 1992
Export Citation:
Assignee:
Chugai Pharmaceutical Co., Ltd.
International Classes:
A61K38/00; A61K39/395; A61P35/00; C07K16/18; C07K16/24; C12N15/09; C07K16/26; C07K16/28; C07K19/00; C12N5/10; C12N15/13; C12P21/08; G01N33/53; G01N33/577; C12R1/91; (IPC1-7): C12N15/09; C07K16/18; C07K19/00; C12N5/10; C12P21/08
Domestic Patent References:
| JP62296890A | ||||
| JP6147500A |
Other References:
【文献】欧州特許出願公開413908(EP,A2)
【文献】欧州特許出願公開409607(EP,A2)
【文献】欧州特許出願公開409607(EP,A2)
Attorney, Agent or Firm:
Takashi Ishida (4 others)