Title:
CHIMERIC RECEPTOR AND METHOD FOR SCREENING LIGAND OR INHIBITOR FOR THE RECEPTOR
Document Type and Number:
Japanese Patent JP2006180705
Kind Code:
A
Abstract:
To provide a chimeric receptor comprising an extracellular region derived from a membrane type guanylate cyclase and an intracellular region derived from a receptor other than the membrane type guanylate cyclase and to provide a method for screening a ligand and inhibitor of the membrane type guanylate cyclase by utilizing the receptor and to provide a substance obtained by the method.
By constructing a human NPR-A/mouse G-CSFR chimeric receptor expression vector and transferring the vector into mouse Ba/F3 cells, a chimeric ANP receptor-expressing cell line highly sensitive to human ANP is successfully obtained. Use of the cell makes it possible to screen the ligand or the inhibitor for the chimeric receptor.
Inventors:
SUZUKI YOSHIYUKI
KOJIMA TETSUO
SATO TAIJI
KOJIMA TETSUO
SATO TAIJI
Application Number:
JP2003060336A
Publication Date:
July 13, 2006
Filing Date:
March 06, 2003
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD
International Classes:
C12N15/09; A61K45/00; A61P7/10; A61P9/04; A61P9/08; A61P9/12; A61P43/00; C07K14/705; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/12; C12Q1/02; G01N33/15; G01N33/50; G01N33/566
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto
Kazunori Hashimoto
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