To obtain the subject inhibiting substance capable of inhibiting the hydrolysis of chlorophyll with chlorophyllase and useful for preventing the yellowlng of leaf vegetables, etc., by using a glucoside originated from a cruciferous flower plant or a milk protein containing a bovine serum albumin and phosphatidylserine.
This chlorophyllase activity-inhibiting substance comprises a glucoside originated from a cruciferous flower plant or a milk protein containing bovine serum albumin and phosphatidylserine. The glucoslde is obtained by grinding the cruclferous flower plant (e.g. Japanese radish) in a 20mM phosphoric acid buffer solution (pH: 7.5) with mixer, centrifuging the ground product, adding methylpentanone to the obtained supernatant, vigorously stirring the mixture, subjecting the mixture to a liquid distribution, collecting the methylpentanone layer, concentrating the collected layer under vacuum, adding methanol to the concentrate, subjecting the mixture to an activated carbon adsorption treatment, eluting the adsorbed substances with ethanol, concentrating the eluate under vacuum, diluting the concentrate into a 30% methanol solution, subjecting the solution to a water immersion type ODS column adsorption treatment, and subsequently eluting the adsorbed active fraction by the linear concentration gradient method of 30-100% methanol. The milk protein containing the bovlne serum albumin and the phosphatidylserine is obtained by adding the bovine serum albumin and the phosphatidylserine to a whey protein which is a milk-originated protein.
KIRIHARA OSAMU
TANI HISANORI
OISHI HIFUMI
