Title:
CLEAVING METHOD FOR ACYL THIOHYDANTOIN AND USE OF THIS METHOD TO C-TERMINAL PEPTIDE SEQUENCING
Document Type and Number:
Japanese Patent JP2544286
Kind Code:
B2
Abstract:
PURPOSE: To provide a method for heightening the cleavage of an acyl thiohydantoin bond to use, e.g. for C-terminal peptide sequencing.
CONSTITUTION: An acyl thiohydantoin is alkylated to form an addition product on the thiohydantoin, and the thiohydantoin containing the addition product is brought to react at the acyl bond under a virtually anhydrous acid condition with a cleaving agent and to be cleaved. The product cleaved in C-terminal amino acid sequencing can be analyzed for identifying the C-terminal amino acid.
Inventors:
BIKUTORIA RII BOIDO
GERARUDO ZON
GERARUDO ZON
Application Number:
JP30161892A
Publication Date:
October 16, 1996
Filing Date:
October 15, 1992
Export Citation:
Assignee:
APURAIDO BAIOSHISUTEMUZU INC
International Classes:
C07K1/02; C07K1/04; C07K1/113; C07K1/12; C07D233/84; C07K7/08; G01N33/68; (IPC1-7): G01N33/68; C07D233/84; C07K1/02
Domestic Patent References:
| JP62156562A | ||||
| JP5501914A |
Other References:
【文献】米国特許5051368(US,A)
【文献】米国特許4935494(US,A)
【文献】米国特許4935494(US,A)
Attorney, Agent or Firm:
Funakashi Eiko