【発明の名称】コバラミン―酸ヒドラジド,その製造法およびコバラミン接合体 - Boehringer Mannheim Geselsyaft Mitt Besilyenktel Haftung

Title:

【発明の名称】コバラミン―酸ヒドラジド,その製造法およびコバラミン接合体

Document Type and Number:

Japanese Patent JPH0717672

Kind Code:

B2

Abstract:

The cobalamin acid hydrazides have the formula (I> B-CO-NH-NH-(R-CO-NH-NH)XH (I) in which B is the radical formed from cobalamins by elimination of a -CONH2 group, in particular the radical of cyanocobalamin, and R is a linking group (spacer), and x is 0 or 1. The cobalamin acid hydrazides of the formula (I) can be used to prepare stable cobalamin conjugates of the formula (III> B-CO-NH-(NH-R-CO-NH)XN=GP (III> in which B, R and x have the abovementioned meaning, and GP is the radical of a glycosyl group-containing protein which is bonded via a glycosyl radical to the -NH-N= group. These cobalamin conjugates, for example the conjugate of the formula B12-d-CO-NH-NH-CO-CH2-(O-CH2-CH2)3-O-CH2-CO-NH-N = PODox, are very suitable for use in immunoassays, especially for the determination of cyanocobalamin.

Inventors:

Erasmus Hoover
Josef Ditzkhoff
Christian Klein
Konrad Kyurtswinger

Application Number:

JP263590A

Publication Date:

March 01, 1995

Filing Date:

January 11, 1990

Export Citation:

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Assignee:

Boehringer Mannheim Geselsyaft Mitt Besilyenktel Haftung

International Classes:

C07H23/00; C07K14/00; C07K14/795; G01N33/531; G01N33/53; G01N33/82; (IPC1-7): C07H23/00; C07K14/795; G01N33/53

Attorney, Agent or Firm:

Toshio Yano (2 outside)

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