Title:
補酵素A依存性アルデヒドデヒドロゲナーゼ経路によるポリヒドロキシアルカノエート産生
Document Type and Number:
Japanese Patent JP4628103
Kind Code:
B2
Abstract:
The present invention provides a method of producing polyhydroxyalkanoate (PHA) polymers comprising at least one hydroxyacyl monomer selected from the group consisting of 3-hydroxypropionate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate, 5-hydroxyvalerate, and 3-hydroxyhexanoate, comprising: expressing in an organism genes encoding a polyhydroxyalkanoate (PHA) synthase and a CoA-dependent aldehyde dehydrogenase, feeding a chemical that is converted by the organism to at least one hydroxyacyl-CoA monomer by an enzyme-catalysed pathway that includes the CoA-dependent aldehyde dehydrogenase activity and allowing polymerisation of the at least one hydroxyacyl-CoA monomer by the PHA synthase to form PHA polymers, and recovering the thus-produced PHA polymers, wherein the organism is produced from a wild-type organism having an endogenous CoA-dependent aldehyde dehydrogenase gene which is modified by gene mutation to increase CoA-dependent aldehyde dehydrogenase activity, or wherein the organism is a recombinant organism in which a gene is recombinantly expressed to encode the CoA-dependent aldehyde dehydrogenase. The present invention also provides a recombinant organism for use in the foregoing method. The present invention also provides a method comprising the step of amplifying a DNA sequence encoding the eutE gene from an E. coli genome using the following oligonucleotides primers: 5' GGT GGT ACC TTA AGA GGA GGT TTT TAT GAA TCA ACA GGA TAT TGA ACA-3' (SEQ ID NO: 1) and 5'-GGT GCG GCC GCT TAA ACA ATG CGA AAC GCA TCG-3' (SEQ ID NO: 2).
Inventors:
Scalary, Frank A.
Application Number:
JP2004536287A
Publication Date:
February 09, 2011
Filing Date:
September 12, 2003
Export Citation:
Assignee:
Metabolics, Incorporated
International Classes:
C12P7/62; C12N1/21; C12N9/02; C12N15/09; C12N15/52
Foreign References:
| WO2002008428A1 | ||||
| WO2000008198A1 | ||||
| WO2000011188A1 |
Other References:
FEMS Microbiology Reviews,1989年,Vol.63,p.223-234
Journal of Bacteriology,1980年,Vol.144, No.1,p.179-184
Applied and Environmental Microbiology,1999年,Vol.65, No.11,p.4973-4980
Journal of Bacteriology,1980年,Vol.144, No.1,p.179-184
Applied and Environmental Microbiology,1999年,Vol.65, No.11,p.4973-4980
Attorney, Agent or Firm:
Yoshio Kawaguchi
Chihiro Watanabe
Katsuma Osaki
Mitsuaki Tsubokura
Chihiro Watanabe
Katsuma Osaki
Mitsuaki Tsubokura