Title:
DE NOVO SYNTHESIZED COMBINATORIAL NUCLEIC ACID LIBRARIES
Document Type and Number:
Japanese Patent JP2023087685
Kind Code:
A
Abstract:
To provide methods for generating highly accurate nucleic acid libraries encoding predetermined variants of a nucleic acid sequence.SOLUTION: Provided herein is a method comprising the steps of: (a) providing predetermined sequences for a plurality of polynucleotides, where the predetermined sequences have a first distribution value that is preselected and is not uniform; b. providing a machine instruction to randomly generate a set of nucleic acid sequence from a single reference sequence; c. calculating a second distribution value from the randomly generated set of nucleic acid sequences; d. determining whether the second distribution value matches the first distribution value or not; e. synthesizing a variant nucleic acid library comprising a plurality of polynucleotides encoded by the randomly generated set of nucleic acid sequences when the first and second distribution values match.SELECTED DRAWING: Figure 22
Inventors:
ANTHONY COX
CHEN SIYUAN
CHARLES LEDOGAR
DOMINIQUE TOPPANI
CHEN SIYUAN
CHARLES LEDOGAR
DOMINIQUE TOPPANI
Application Number:
JP2023011285A
Publication Date:
June 23, 2023
Filing Date:
January 27, 2023
Export Citation:
Assignee:
TWIST BIOSCIENCE CORP
International Classes:
C12N15/13; C12N15/10; C40B40/08
Attorney, Agent or Firm:
Kiyohara International Patent Office