Title:
生殖細胞系伝達能力を有する胚性幹(ES)細胞のインビトロの誘導および培養のための組成物
Document Type and Number:
Japanese Patent JP4408371
Kind Code:
B2
Abstract:
The present invention is directed to a method of deriving pluripotent embryonic stem cells from mouse blastocysts or from primordial germ cells from a post-implantation mouse embryo, or of maintaining or growing pluripotent embryonic stem cells from a mouse, or of expanding human hematopoietic stem cells or human hematopoietic precursor cells. The methods include the step of cultivating the stem cells or precursor cells for at least one passage in a culture medium preconditioned by the rabbit fibroblast cell line Rab9 (ATCC catalogue CRL1414) and containing less than 0.1 ng/ml Leukemia Inhibitory Factor (LIF).
Inventors:
Scone yans, look
Application Number:
JP2003554874A
Publication Date:
February 03, 2010
Filing Date:
December 20, 2002
Export Citation:
Assignee:
THROMBOGENICS N.V.
International Classes:
C12N5/07; A01K67/027; C07K14/47; C07K14/54; C12N1/19; C12N5/0735; C12N5/0789; C12N15/09; A61K35/12
Domestic Patent References:
| JP10052263A | ||||
| JP2001017163A |
Other References:
Oncogene, vol.16, pp.3189-3196 (1998)
Attorney, Agent or Firm:
Kuro Fukami
Toshio Morita
Yoshihei Nakamura
Yutaka Horii
Hisato Noda
Masayuki Sakai
Nobuo Arakawa
Toshio Morita
Yoshihei Nakamura
Yutaka Horii
Hisato Noda
Masayuki Sakai
Nobuo Arakawa