Title:
ＤＮＡ結合ドメインと切断ドメインとを連結するための組成物
Document Type and Number:
Japanese Patent JP6840796
Kind Code:
B2
Abstract:
Disclosed herein are compositions for linking DNA-binding domains and cleavage domains to form nucleases, for example nucleases with altered target site separation (gap) preferences as compared to conventional linkers. Also described are fusion proteins comprising these linkers. The disclosure also provides methods of using these fusion proteins and compositions thereof for targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined region of interest in cells. Thus, in one aspect, described herein are amino acid sequences linking a DNA-binding domain to a cleavage domain. The linker may replace one or more residues of the DNA-binding domain and/or cleavage domain.
Inventors:
David Passion
Leysin
Leysin
Application Number:
JP2019133572A
Publication Date:
March 10, 2021
Filing Date:
July 19, 2019
Export Citation:
Assignee:
Sangamo Therapeutics, Incorporated
International Classes:
C12N15/09; A61K31/7088; A61K38/46; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16
Domestic Patent References:
| JP2011521643A |
Other References:
Mol.Cells (May 2013) vol.35, no.5, p.359-370
Nat. Biotechnol. (Apr 2014) vol.32, no.6, p.569-576
Nat. Biotechnol. (Apr 2014) vol.32, no.6, p.577-582
Mol. Cell. Biol. (2001) vol.21, no.1, p.289-297
Mol. Ther. (2009) vol.17, no.1, p.104-111
Mol. Ther. Nucleic Acids (Apr 2013) vol.2, e88
Nat. Biotechnol. (Apr 2014) vol.32, no.6, p.569-576
Nat. Biotechnol. (Apr 2014) vol.32, no.6, p.577-582
Mol. Cell. Biol. (2001) vol.21, no.1, p.289-297
Mol. Ther. (2009) vol.17, no.1, p.104-111
Mol. Ther. Nucleic Acids (Apr 2013) vol.2, e88
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto