Title:
ｉｎ－ｓｉｔｕキャリブレーションを用いるゲル電気泳動用の組成物及び方法
Document Type and Number:
Japanese Patent JP7082467
Kind Code:
B2
Abstract:
The invention relates to, among other things, a method for performing electrophoresis with in-situ calibration. The method includes combining a volume of a test sample with a volume or quantity of a calibrating sample to form a final volume, where the volume of the calibrating sample includes a known concentration of a calibrator and the final volume includes a known ratio of test sample to calibrating sample. The method also includes depositing a loading fraction in a receiving well of an electrophoretic gel, in which the loading fraction is a fraction of the final volume, and separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane. The method also includes detecting the calibrator and separated components of the test sample within the common separation lane and measuring the level of the calibrator and separated components of the test sample based on the detecting, thereby performing electrophoresis with in-situ calibration.
Inventors:
Guadagno, Philip
Summers, Erin
Summers, Erin
Application Number:
JP2017201797A
Publication Date:
June 08, 2022
Filing Date:
October 18, 2017
Export Citation:
Assignee:
Helena Laboratories Corporation
International Classes:
G01N27/26; G01N27/447; G01N33/559
Domestic Patent References:
| JP9508209A | ||||
| JP2005181299A | ||||
| JP2011039002A | ||||
| JP2009168819A | ||||
| JP2008232927A | ||||
| JP10507514A | ||||
| JP4232850A | ||||
| JP2007024610A |
Foreign References:
| US20040106153 |
Other References:
須藤加代子,”Immunofixation electrophoresis”,臨床検査,1979,Vol.23,No.9,p.881-887
大竹皓子,”Immunofixation電気泳動法”,検査と技術,1979,Vol.7,No.4,p.339-346
大竹皓子,”Immunofixation電気泳動法”,検査と技術,1979,Vol.7,No.4,p.339-346
Attorney, Agent or Firm:
Osamu Yamamoto
Toru Miyamae
Yamaguchi Akiko
Toru Miyamae
Yamaguchi Akiko