Title:
インビトロウイルスゲノム工学のための組成物及びその方法
Document Type and Number:
Japanese Patent JP7133671
Kind Code:
B2
Abstract:
The present disclosure relates to a method of in vitro engineering of nucleic acids. This disclosure further relates to in vitro engineering of viral genomes and to the improvement of viral properties by in vitro genomic engineering of viral genomes. Specifically, the disclosure relates to in vitro viral genomic digestion using RNA-guided Cas9, the assembly of a recombinant genome by the insertion of a DNA or RNA fragment into the digested viral genome and transformation of a host cell with the recombinant genome. This method also related to in vitro engineering for error correction of nucleic acids.
Inventors:
Caddy Kyle Sea.
Barb E. Magda
Dipetrillo Christen Gee.
Barb E. Magda
Dipetrillo Christen Gee.
Application Number:
JP2021024982A
Publication Date:
September 08, 2022
Filing Date:
February 19, 2021
Export Citation:
Assignee:
C3J Therapeutics Inc.
International Classes:
C12N7/01; C12N15/09; C12N15/31; C12N15/55
Foreign References:
| WO2006137847A2 |
Other References:
Polish Journal of Microbiology,2014年,vol.63, no. 2,p.137-145, entered STN: 14 Aug 2014
Current Pharmaceutical Biotechnology,2008年,vol.9,p.261-266
Current Pharmaceutical Biotechnology,2008年,vol.9,p.261-266
Attorney, Agent or Firm:
Shimizu Hatsushi
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto