Title:
誘導されたエキソソームを含む皮膚再生及び創傷治癒用組成物
Document Type and Number:
Japanese Patent JP7249693
Kind Code:
B2
Abstract:
Disclosed is method for producing exosomes having skin regeneration and wound healing effects, the method comprising steps of: providing ultrasound stimulation directly or indirectly to cells; culturing a mixture of the cells and a medium for a predetermined time; and isolating exosomes from the mixture, wherein providing the stimulation directly to the cells comprises applying ultrasound stimulation to the medium containing the cells, and providing the stimulation indirectly to the cells comprises applying ultrasound stimulation to the medium not containing the cells and then mixing the medium and the cells. This method for producing exosomes makes it possible to obtain exosomes having skin regeneration and wound healing effects not only from stem cells and progenitor cells that are difficult to isolate and multiply, but also from somatic cells that may be easily obtained and maintained, in high yield within a short time by ultrasound treatment that is a simple process. The amount and number of various factors contained in the obtained exosomes are also large, and the exosomes and a composition containing the same contain increased amounts of various factors capable of inducing skin regeneration and wound healing. The exosomes have a phospholipid-based membrane structure, and thus may easily penetrate the skin and deliver substance with high efficiency, thus effectively inducing skin regeneration and wound healing. Moreover, the exosomes are safe and have the advantage of having no side-effects occurring due to the use of synthetic drugs.
Inventors:
Lee, Young Soon
Application Number:
JP2021516777A
Publication Date:
March 31, 2023
Filing Date:
September 19, 2019
Export Citation:
Assignee:
STEMON INC.
International Classes:
C12N5/071; A61K35/12; A61P17/02; C12N1/02; C12N13/00
Foreign References:
| WO2017155166A1 | ||||
| WO2017123022A1 | ||||
| US20180177828 |
Attorney, Agent or Firm:
Shin Shin