Title:
COMPOUND AND METHOD FOR SITE-SPECIFIC PHOTOAFFINITY LABELLING OF BIOMOLECULE
Document Type and Number:
Japanese Patent JP3876341
Kind Code:
B2
Abstract:
PURPOSE: To provide novel heterotrifunctional compounds as photoaffinity labelling of biomolecules which have water-soluble groups before and after labelling of biomolecule and whose solubility is kept so that the labelling molecules protect free into the solution after immobilized on the surface of the biomolecules.
CONSTITUTION: By treating N-(p-azidobenzensulfonyl) N'-(3-carboxypropionyl) cysteamine with N-hydroxysuccinimide, a heterotrifunctional compound of formula I {wherein X1 represents carbonyl or sulfonyl; Y represents hydrogen, Y', X1Y' (wherein Y' represents hydroxyl, amino or alkoxy); R represents a group of formula II [wherein X2 represents disulfide or methylene; R1 represents amino (-NH-R2), R2 represents hydrogen, carboxyalkanoyl, cyclohexandion derivative (-NH-cyclohexendion), carboxyl derivative or the like; (n') and (n") are each of 1 to 6]}, for example N-(p-azidobenzensulfonyl) N'-(3- succinimidyloxycarboxypropionyl)cysteamine, is obtained.
Inventors:
Ricardo Barner
Walter Hoover
Joseph Hubeskell
Jürg Hurst
Daniel Sclatter
Walter Hoover
Joseph Hubeskell
Jürg Hurst
Daniel Sclatter
Application Number:
JP17042093A
Publication Date:
January 31, 2007
Filing Date:
July 09, 1993
Export Citation:
Assignee:
MICRONAS GmbH
International Classes:
C07C247/16; C07C247/18; C07D207/416; C07D213/18; C07K1/00; C07K1/113; C07K14/00; C07K16/00; C07K19/00; G01N33/532; C07D207/40; (IPC1-7): C07C247/16; C07D213/18; C07K15/22
Other References:
Biochem.J.,223(2),p.519-26(1984)
Attorney, Agent or Firm:
Hideto Asamura
Hajime Asamura
Naganuma Akio
Hajime Asamura
Naganuma Akio