PURPOSE: To concentrate and purity the title substance, especially a fine amount of an unstable active substance, with simple operations and apparatus in a state capable of being readily scaled up, by using specific mixed organic solvents and allowing the phase separation of the solvents to occur by the change of the temperature of the solvents to separate the antibiotic contained in the solvents.
CONSTITUTION: The objective antibiotic is concentrated and purified by changing the temperature of a solution of the antibiotic in mixed organic solvents causing the phase separation of the solvents by the change of the temperature and subsequently separating the separated phases from each other. The phase separation is performed at a temperature between the upper critical perfectly melting temperature of the solution and the perfectly coagulating point thereof. When precipitates other than the objective antibiotic are produced in a system wherein an organic solvent such as acetonitrile, an acid, a base, etc., are added to a cultured solution containing the objective antibiotic, etc., the precipitates are removed by a centrifugal separation method to recover a supernatant. Acetonitrile-water system, butyric acid-water system, methylpropionic acid-water system, etc., is used for the used organic solvent solution and fosmicin, adriamycin, etc., is used as the antibiotic.
Takayama, Seiji
Shibai, Hiroshiro
Ishii, Koichi
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