To provide a method for more accurately constructing a superimposed image by easily correcting distortion or the like in acquiring thin layer slices when superimposing two-dimensional images obtained by imaging a plurality of thin layer slices obtained from one embedding sample.
A superimposed image construction method includes a step (a) of creating an embedding sample in which a specimen to be examined and a marker are embedded, a step (b) of creating two or more thin layer slices by slicing the embedding sample after the step (a), a step (c) of capturing two-dimensional images of the created thin layer slices after the step (c), and a step (d) of constructing an image by superimposing the captured two-dimensional images with the markers in the two-dimensional images as criteria after the step (c).
JPH0835921A | 1996-02-06 | |||
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US20060051736A1 | 2006-03-09 |
Masatake Shiga
Suzuki Mitsuyoshi
Tadao Takashiba
Hiroshi Masui
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