PURPOSE: To convert adenosine monophosphate (AMP) essentially completely to adenosine triphosphate (ATP), stably for a long period, by using an immobilized enzyme and adjusting the mixing ratio of AMP to ATP to a specific state.
CONSTITUTION: An enzyme capable of converting AMP to adenosine diphosphate (ADP) such as adenylate kinase and an enzyme capable of converting ADP to ATP such as acetate kinase are prepared by microorganisms having an optimum proliferation temperature of 50W85°C. Both enzymes are immobilized separately, and the immobilized enzymes are used in combination for the conversion of AMP via ADP to ATP. In the above conversion process, the concentration of ATP is adjusted to a level satisfying the formula [AMP in the formula is concentration (mM) of AMP, ATP is concentration (mM) of ATP, γ is the ratio of the activity of the enzyme converting ADP to ATP to that of the enzyme converting AMP to ADP, and is ≥1].
KONDOU HITOSHI
NAKAJIMA HIROSHI
IWASAKI TATSUO
RIKAGAKU KENKYUSHO
UNITIKA LTD
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