Title:
タンパク質およびその基質への、共有結合による機能性基の係留
Document Type and Number:
Japanese Patent JP5214244
Kind Code:
B2
Abstract:
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
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Inventors:
Dargins, Ardis
Ensel, Lancepie.
Tony, Johnson
Claubert, Dieter
Ross, George Buoy.
McDougall, Mark
Wood, Keith Buoy.
Wood, Monica G.
Jim Plitch, Chad
Ensel, Lancepie.
Tony, Johnson
Claubert, Dieter
Ross, George Buoy.
McDougall, Mark
Wood, Keith Buoy.
Wood, Monica G.
Jim Plitch, Chad
Application Number:
JP2007523888A
Publication Date:
June 19, 2013
Filing Date:
July 29, 2005
Export Citation:
Assignee:
Promega Corporation
International Classes:
C12N15/09; C07K1/22; C07K14/47; C07K19/00; C12N9/10; C12N9/14; C12N11/00; C12Q1/02; C12Q1/26; C12Q1/34; C12Q1/37; C12Q1/48; G01N21/78; C12N5/10
Domestic Patent References:
| JP2003530365A |
Foreign References:
| WO2001060415A1 |
Other References:
Tetrahedron,2002年,58,p.3785-92
J. Heterocyclic Chem.,1998年,35,p.675-86
Tetrahedron,1999年,55,p.12939-56
2nd Symposium on Biological Imaging,2003年 5月30日,p.16
J. Bacteriol.,1987年,169(9),p.4049-54
J. Heterocyclic Chem.,1998年,35,p.675-86
Tetrahedron,1999年,55,p.12939-56
2nd Symposium on Biological Imaging,2003年 5月30日,p.16
J. Bacteriol.,1987年,169(9),p.4049-54
Attorney, Agent or Firm:
Koichi Tsujii
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Satsuki Ichikawa
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Satsuki Ichikawa