Title:
ＣＹ０１菌株由来の菌体外多糖を含有する凍結保護剤
Document Type and Number:
Japanese Patent JP6768959
Kind Code:
B2
Abstract:
The present invention relates to an exopolysaccharide derived from Pseudoalteromonas sp. strain CY01 (KCTC 12867BP) which is a novel strain living in the polar regions, and to a composition for cryoprotection of cells, which contains the exopolysaccharide. The exopolysaccharide of the present invention has an excellent ability to cryoprotect cells, and shows no cytotoxicity. Thus, the inventive exopolysaccharide can substitute for conventional cryoprotective agents that show cytotoxicity when used at high concentrations.
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Inventors:
Im, john han
Kim, Il-chan
Han, Sejong
Yun, Uijeong
Lee, Hong Kum
Kim, Sung Jin
Kang, Pill-Son
Kim, Jung Eun
Kim, taikyon
Park, Haju
Son, Jin Heng
Kim, Minju
Hong, Jumi
Cho, Don-Gyu
Kim, Il-chan
Han, Sejong
Yun, Uijeong
Lee, Hong Kum
Kim, Sung Jin
Kang, Pill-Son
Kim, Jung Eun
Kim, taikyon
Park, Haju
Son, Jin Heng
Kim, Minju
Hong, Jumi
Cho, Don-Gyu
Application Number:
JP2019530634A
Publication Date:
October 14, 2020
Filing Date:
December 18, 2017
Export Citation:
Assignee:
ALTEROBIOTECH INC.
International Classes:
C12P19/04; C08B37/00; C12N1/04; C12N1/20
Foreign References:
| KR1020090050191A | ||||
| KR20160138758A | ||||
| US5071741 |
Other References:
KIM, S. J. et al.,Cryoprotective Properties of Exopolysaccharide (P-21653) Produced by the Antarctic Bacterium, Pseudoalteromonas arctica KOPRI 21653,The Journal of Microbiology,2007年12月,Vol.45, No.6,p.510-514
MANCUSO, C. et al.,Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea ice Bacterium Grown in Batch Culture,APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2005年 7月,Vol.71, No.7,p.3519-3523
MANCUSO, C. et al.,Chemical Characterization of Exopolysaccharides from Antarctic Marine Bacteria,Microbial Ecology,2005年 7月28日,Vol.49,p.578-589
LIU, Sheng-Bo et al.,Structure and Ecological Roles of a Novel Exopolysaccharide from the Arctic Sea Ice Bacterium Pseudoalteromonas sp. Strain SM20310,Applied and Environmenttal Microbiology,2013年 1月,Vol.79, No.1,p.224-230
MANCUSO, C.A. et al.,Production of exopolysaccharides by Antarctic marine bacterial isolates,Journal of Applied Microbiology,2004年,Vol.96,p.1057-1066
ROUGEAUX, H. et al.,Novel bacterial exopolysaccharides from deep-sea hydrothermal vents,Carbohydrate Polymers,1996年,Vol.31,p.237-242
AI-NAHAS, M. O. et al.,Characterization of an exopolysaccharide-producing marine bacterium, isolate Pseudoalteromonas sp. AM,African Journal of Microbiology Research,2011年10月16日,Vol.5, No.22,p.3823-3831
QIN, G. et al.,Structural charracterization and ecological roles of a novel exopolysaccharide from the deep-sea psychrotolerant bacterium Pseudoalteromonas sp. SM9913,Microbiology,2007年,Vol.153,p.1566-1572
MANCUSO, C. et al.,Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea ice Bacterium Grown in Batch Culture,APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2005年 7月,Vol.71, No.7,p.3519-3523
MANCUSO, C. et al.,Chemical Characterization of Exopolysaccharides from Antarctic Marine Bacteria,Microbial Ecology,2005年 7月28日,Vol.49,p.578-589
LIU, Sheng-Bo et al.,Structure and Ecological Roles of a Novel Exopolysaccharide from the Arctic Sea Ice Bacterium Pseudoalteromonas sp. Strain SM20310,Applied and Environmenttal Microbiology,2013年 1月,Vol.79, No.1,p.224-230
MANCUSO, C.A. et al.,Production of exopolysaccharides by Antarctic marine bacterial isolates,Journal of Applied Microbiology,2004年,Vol.96,p.1057-1066
ROUGEAUX, H. et al.,Novel bacterial exopolysaccharides from deep-sea hydrothermal vents,Carbohydrate Polymers,1996年,Vol.31,p.237-242
AI-NAHAS, M. O. et al.,Characterization of an exopolysaccharide-producing marine bacterium, isolate Pseudoalteromonas sp. AM,African Journal of Microbiology Research,2011年10月16日,Vol.5, No.22,p.3823-3831
QIN, G. et al.,Structural charracterization and ecological roles of a novel exopolysaccharide from the deep-sea psychrotolerant bacterium Pseudoalteromonas sp. SM9913,Microbiology,2007年,Vol.153,p.1566-1572
Attorney, Agent or Firm:
Hiroyuki Nagai
Yukitaka Nakamura
Satoru Asakura
Mari Asano
Hiroshi Sorimachi
Yukitaka Nakamura
Satoru Asakura
Mari Asano
Hiroshi Sorimachi