CULTIVATION OF PRIMATE EMBRYONIC STEM CELLS

Title:

CULTIVATION OF PRIMATE EMBRYONIC STEM CELLS

Document Type and Number:

Japanese Patent JP2016208999

Kind Code:

A

Abstract:

PROBLEM TO BE SOLVED: To provide methods for culturing human embryonic stem cells without the need for feeder cells or for exposure of a culture medium to feeder cells.SOLUTION: Stem cells are cultured in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferrin, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than just a feeder layer culture medium.SELECTED DRAWING: None

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Inventors:

THOMSON JAMES A
MARK LEVENSTEIN

Application Number:

JP2016186252A

Publication Date:

December 15, 2016

Filing Date:

September 23, 2016

Export Citation:

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Assignee:

WICELL RES INST INC
WISCONSIN ALUMNI RES FOUND

International Classes:

C12N5/0735; C12N1/00

Domestic Patent References:

JP2003525625A

2003-09-02

Foreign References:

WO2001066697A2	2001-09-13
WO2003020920A1	2003-03-13

Other References:

TRENDS IN GENETICS, vol. Vol. 20, No. 11, JPN6017031690, 2004, pages p. 563-569
TRENDS IN GENETICS, vol. Vol. 20, No. 11, JPN6017031690, 2004, pages p. 563-569
AMIT M, BIOLOGY OF REPRODUCTION, vol. V70, JPN5007017295, 19 November 2003 (2003-11-19), US, pages 37 - 845
LI Y, BIOTECHNOLOGY AND BIOENGINEERING, vol. V91 N6, JPN5007017296, 20 September 2005 (2005-09-20), US, pages 88 - 698

Attorney, Agent or Firm:

Takaki Nishijima
Disciple Maru Ken
Shinichiro Tanaka
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Satsuki Ichikawa
Hironobu Hattori

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