To obtain the subject cell for the treatment of leukemia, etc., which can be transplanted without anaesthetizing and even to a patient ill in systemic conditions by culturing human hematopoietic stem cells of a specific phenotype in the presence of hematopoietic stem cell proliferation factors by liquid culture in an adhesive culturing vessel.
Human hematopoietic stem cells separated by an ordinary method from cord blood collected from a healthy volunteer is characterized in that its phenotype is CD34+ and c-kit-. The human hematopoietic stem cells are cultured by liquid culture in the presence of hematopoietic stem cell proliferation factors consisting of human flt-3-ligand, human interleukin-6 and human interleukin-7 in an adhesive culture vessel made of polystyrene, etc., to obtain the objective hematopoietic stem cells. When the cells are used, there is no need of anaesthetizing, which is required on apheresis for collecting self stem cells, transplant operation can be performed on a patient whose systemic conditions or organ functions are not good, and the speed of hematopoietic recovery after transplant operation is high. Therefore, the cells are useful for the treatment of neoplstic disease such as leukemia and nonneoplastic disease such as serious aplastic anemia, etc.
YAMANISHI KAZUYA
ADACHI SHOICHI
IKEHARA SUSUMU
