Title:
ヒト胚幹細胞を迅速に拡大するための培養系
Document Type and Number:
Japanese Patent JP5792428
Kind Code:
B2
Abstract:
This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.
Inventors:
Mandalam Rankmer
Fuchunhui
Gold Joseph Dee.
Carpenter Melissa Kay.
Fuchunhui
Gold Joseph Dee.
Carpenter Melissa Kay.
Application Number:
JP2009271501A
Publication Date:
October 14, 2015
Filing Date:
November 30, 2009
Export Citation:
Assignee:
Asterias BioTherapeutics, Inc.
International Classes:
C12N5/0735; A61K35/12; C12N15/09; A61P43/00; C12N1/00; C12N5/071; C12N5/077; C12N5/079; C12N5/0793; C12N5/10; C12N15/10
Domestic Patent References:
| JP200117163A |
Foreign References:
| WO1999053021A1 |
Attorney, Agent or Firm:
Naoto Uchida