To provide a fluorescent dye having a favorable permeability through a cell membrane which is used for detecting a nucleic acid.
The present invention discloses the manufacture and the use of a fluorescent dye used for dying a nucleic acid, wherein the dye comprises a substituted benzazolium ring system having at least one substituent of a saturated or unsaturated cyclic substituent bonded to a pyridinium or quinolinium ring system by a methine crosslinkage. By virtue of an excellent fluorescence property of the dye which forms a complex with a nucleic acid, the dye can be used for detecting an oligonucleotide and the nucleic acid in a cell, gel or solution. By comprising a cyclic substituent, the permeability of the dye into cells and gels in a wide range can be improved and consequently, the detection of the nucleic acid by the dye can be improved. The combination of the dye with an additional dye enables the perfection of a cell membrane, and also enables the gram-sign or the structure and function of a cell to be analyzed.
WELLS K SAM
MILLARD PAUL J
YUE STEPHEN T
ROTH BRUCE L
| JPS4943321B1 | 1974-11-20 | |||
| JPS62242933A | 1987-10-23 | |||
| JPH02191674A | 1990-07-27 | |||
| JPH02245200A | 1990-09-28 | |||
| JPH0366763A | 1991-03-22 |
| US3639127A | 1972-02-01 | |||
| WO1993006482A1 | 1993-04-01 |
Takaaki Yasumura
Natsuki Morishita
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