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Title:
D-LACTATE DEHYDROGENASE AND ITS PRODUCTION
Document Type and Number:
Japanese Patent JP3773283
Kind Code:
B2
Abstract:

PURPOSE: To produce a D-lactate dehydrogenase, excellent in stability and having high specific activities.
CONSTITUTION: This D-lactate dehydrogenase having the following physicochemical properties by culturing a Leuconostoc lactis SHO47 strain, etc.: (1) action: catalyzing the reaction of the following reactional formula: D-lactic acid + NAD+ pyruvic acid + NADH + H+; (2) stability: having ≥90% residual activities after allowing thereof to stand in a 100mM phosphoric acid buffer solution (pH7.0) in the presence of glycerol or a bovine blood serum albumin at ambient temperature for 1 month; (3) specific activity: ≥2000U/mg protein (30°C measurement temperature) in reducing reaction for pyruvic acid; (4) about 80000-85000 molecular weight [measured by the gel filtration chromatography with Sephadex G-100 (R)]; (5) 7-8 optimum pH range (the reducing reaction for the pyruvic acid); (6) 5-11 stable pH region and (7) the range of optimum action temperature: within the range of 20-50°C (phosphoric acid buffer solution at pH7.0).


Inventors:
Kazuyuki Uchida
Kawahara Kazue
Roy Russell
Hitoshi Kondo
Hitomi Ohara
Masato Yahata
Application Number:
JP15647095A
Publication Date:
May 10, 2006
Filing Date:
June 22, 1995
Export Citation:
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Assignee:
UNITIKA LTD.
TOYOTA JIDOSHA KABUSHIKI KAISHA
International Classes:
C12N9/04; C12R1/01; (IPC1-7): C12N9/04; //(C12N9/04; C12R1:01)
Other References:
Biochimie, 55 (1973) p.1047-1056
Microbios, 9 (1974) p.199-215
Int. J. Biochem. 18 [1] (1986) p.31-38
J. Dairy Res. 59 (1992) p.359-367
Attorney, Agent or Firm:
Toshio Nishizawa