To provide a method for producing an optically active β-hydroxyamino acid, a new D-phenylserine deaminase, a method for producing the enzyme and a method for producing L-β-hydroxyamino acid by decomposing only D-isomer of DL-β-hydroxyamino acid using the enzyme.
D-3-Phenylserine deaminase produced by a bacterium belonging to the genus Arthrobacter, Achromobacter, Bordetella, Stenotrophomonas or Pseudomonas is purified and its enzymatic properties are elucidated. A gene encoding the enzyme is cloned to Escherichia coli using a part of internal amino acid sequence of the purified enzyme and expressed. The D-3-phenylserine deaminase is produced by the obtained transformant. It is found that the enzyme is made to act on DL-β-hydroxyamino acid and an optical resolution is carried out to produce L-β-hydroxyamino acid.
NAGATA SHINJI
YAMAMOTO HIROAKI
KIMOTO KUNIHIRO
DAICEL CHEM
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Shinichi Watanabe
Masato Ozeki
Kazuya Kawamoto