Title:
穀物からの4群主要アレルゲンのDNA配列および組換え製剤
Document Type and Number:
Japanese Patent JP4960102
Kind Code:
B2
Abstract:
DNA molecules (I), corresponding to the sequence of the major allergens of cereal pollen are new. (I) have sequences (1; 1603 nucleotides (nt)); (3; 1644 nt); (5; 1608 nt); (7; 1603 nt) or (9; 1603 nt), all reproduced. DNA molecules (I), corresponding to the sequence of the major allergens of cereal pollen comprise sequences comprising 1603 nucleotides (SEQ ID No.:1) (nt)); 1644 nucleotides (SEQ ID No.:3; ); 1608 nucleotides (SEQ ID No.:5; ); 1603 nucleotides (SEQ ID No.:7; ) or 1603 nucleotides (SEQ ID No.:9; ). Independent claims are also included for: (1) DNA (Ia) that hybridizes to (I) under stringent conditions and is derived from DNA of Poaceae; (2) DNA (Ib) that encodes a polypeptide that is immunologically cross-reactive with the major allergens Sec c 4 ( Secale cereale); Hor v 4 ( Hordeum vulgare) or Tri a 4 ( Triticum aestivum) and derived from Poaceae; (3) DNA fragment, or combination of fragments, of (I), (Ia) or (Ib) that encodes an immunomodulatory T-cell reactive fragment (X) of Group 4 Poaceae allergens; (4) a DNA corresponding to any of the new DNAs or fragments that encodes an (X) but is modified by targeted mutation of individual codons, deletion or addition; (5) recombinant DNA expression vector or cloning system containing any of the new DNAs linked to expression control sequences; (6) host organism transformed with any of the new DNAs or the vector of (5); (7) preparing a polypeptide (II) encoded by the new DNAs by culturing the organisms of (6); and (8) (II) having any of the sequences, comprising 518 amino acids (SEQ ID No.:2; 520 amino acids (SEQ ID No.:4; ); 518 amino acids (SEQ ID No.:6; ); 518 amino acids (SEQ ID No.:8) or 518 amino acids (SEQ ID No.: 10; ) or their mature forms. ACTIVITY : Antiallergic. No biological data given. MECHANISM OF ACTION : Vaccine.
Inventors:
Fevik, Helmut
Nadi, Andreas
Cromwell, Oliver
Nadi, Andreas
Cromwell, Oliver
Application Number:
JP2006544263A
Publication Date:
June 27, 2012
Filing Date:
December 01, 2004
Export Citation:
Assignee:
Merck Patent Gesellschaft mit beschraenkter Haftung
International Classes:
C12N15/09; A61K31/713; A61K38/00; A61K39/35; A61K39/36; A61P37/08; C07K14/415; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02; A61K38/095
Domestic Patent References:
| JP9504167A | ||||
| JP6509941A |
Foreign References:
| WO2004000881A1 | ||||
| WO2003000898A1 |
Other References:
FISCHER, S. et al.,"Characterization of Phl p 4, a major timothy grass (Phleum pratense) pollen allergen.",JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY,1996年 7月,Vol.98, No.1,P.189-198
STUMVOLL, S. et al.,"Purification, structural and immunological characterization of a timothy grass (Phleum pratense) pollen allergen, Phl p 4, with cross-reactive potential.",BIOLOGICAL CHEMISTRY,2002年 9月,Vol.383, No.9,P.1383-1396
SCHEINER, O. et al.,"cDNA cloning, of the major pollen allergens from timothy grass (Phleum pratense) and rye (Secale cereale). Diagnosis of grass pollen allergy with recombinant allergens.",MOLECULAR BIOLOGY AND IMMUNOLOGY OF ALLERGENS,1993年,P.153-156
STUMVOLL, S. et al.,"Purification, structural and immunological characterization of a timothy grass (Phleum pratense) pollen allergen, Phl p 4, with cross-reactive potential.",BIOLOGICAL CHEMISTRY,2002年 9月,Vol.383, No.9,P.1383-1396
SCHEINER, O. et al.,"cDNA cloning, of the major pollen allergens from timothy grass (Phleum pratense) and rye (Secale cereale). Diagnosis of grass pollen allergy with recombinant allergens.",MOLECULAR BIOLOGY AND IMMUNOLOGY OF ALLERGENS,1993年,P.153-156
Attorney, Agent or Firm:
Kiyoji Kuzuwa
Noriki Mitsuhashi
Takakawara Yoshiko
Noriki Mitsuhashi
Takakawara Yoshiko