Title:
DNA分析方法および分析装置
Document Type and Number:
Japanese Patent JP4945279
Kind Code:
B2
Abstract:
Disclosed is a DNA analysis method and a DNA analyzer whose signal intensity is not lowered even when a material at a higher density is measured. There is supplied dATP, dTTP, dGTP, or dCTP from a dATP solution vessel, a dTTP solution vessel, a dGTP solution vessel, or a dCTP solution vessel, and this causes an extension reaction of a double-stranded DNA immobilized to a bead, to yield pyrophosphoric acid. The pyrophosphoric acid is converted into a redox compound by the actions of a reagent and an enzyme contained in a reaction buffer in a reaction buffer vessel. The redox compound causes a variation in surface potential of a measuring electrode bearing an electrochemically active material immobilized thereto through an insulating molecule, and this variation causes a variation in drain current of a field-effect transistor electrically connected to the measuring electrode. Thus, the extension reaction is detected.
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Inventors:
Yu Ishige
Masao Kamabori
Masao Kamabori
Application Number:
JP2007077294A
Publication Date:
June 06, 2012
Filing Date:
March 23, 2007
Export Citation:
Assignee:
株式会社日立製作所
International Classes:
G01N27/416; C12Q1/68; G01N27/414; C12N15/09
Domestic Patent References:
| JP2003000299A | ||||
| JP2006058020A | ||||
| JP2003527846A | ||||
| JP8500724A | ||||
| JP2001056337A | ||||
| JP2004508833A | ||||
| JP2003262604A | ||||
| JP2005218310A | ||||
| JP54061984A | ||||
| JP2008128803A |
Foreign References:
| WO2003078655A1 | ||||
| WO2005073399A1 | ||||
| WO2007083693A1 | ||||
| WO2005003750A1 | ||||
| WO2001042496A1 |
Other References:
SAKATA Toshiya, MIYAHARA Yuji ,DNA Sequencing Based on Intrinsic Molecular Charges,Angew Chem,ドイツ,2006年 3月27日,Vol.45 No.14,2225-2228
Attorney, Agent or Firm:
Yusuke Hiraki