Title:
【発明の名称】改変型デアセトキシセファロスポリンCシンターゼ(DAOCS)およびX線構造
Document Type and Number:
Japanese Patent JP2002500015
Kind Code:
A
Abstract:
Three-dimensional crystal structure(s) of deacetoxycephalosporin C synthase (DAOCS) are described. The X-ray co-ordinates provide precise 3-dimensional information of amino acids within the structure of DAOCS. Some of these are in complexes with iron and/or substrates. Information from the structures is used to modify enzymes of the cephalosporin biosynthesis pathway including DAOCS, deacetylcephalosporin C synthase DAOC/DACS, such that they accept unnatural substrates (e.g. penicillins G, V) in order to improve the production of beta-lactam antibiotics. The structures may be used to predict the structures of other 2-oxoglutarate dependent enzymes, thereby allowing the design of inhibitors, and new catalysts for the production of e.g. oxidised amino acids/peptides. Specific modifications of amino acid residues are proposed and exemplified.
Inventors:
Shofield, Christopher, Joseph
Baldwin, Jack, Edward
Roach, Peter, Elle.
Lloyd, Matthew, Dee.
Carl, Harros
Anderson, Inger
Hardew, Janos
Turveys Shervan Sheltinga, Anque, Es.
Valgard, Karin
Ramaz Whammy, S.
Baldwin, Jack, Edward
Roach, Peter, Elle.
Lloyd, Matthew, Dee.
Carl, Harros
Anderson, Inger
Hardew, Janos
Turveys Shervan Sheltinga, Anque, Es.
Valgard, Karin
Ramaz Whammy, S.
Application Number:
JP2000526650A
Publication Date:
January 08, 2002
Filing Date:
December 24, 1998
Export Citation:
Assignee:
Isis Innovation Limited
International Classes:
C12N15/09; A61K45/00; A61P1/12; A61P19/02; C07D501/10; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12N9/02; C12P35/00; (IPC1-7): C12N15/09; A61K45/00; A61P1/12; A61P19/02; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12P35/00
Attorney, Agent or Firm:
Yusuke Hiraki (1 person outside)