Title:
【発明の名称】ピリミジンデオキシリボヌクレオシド産生のためのベクター、細胞および方法
Document Type and Number:
Japanese Patent JP2003504029
Kind Code:
A
Abstract:
Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.
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Inventors:
David, Martin, Anderson
Lynn, Liu
Sergey, Podkobilov
Bao Ming, Wang
Lynn, Liu
Sergey, Podkobilov
Bao Ming, Wang
Application Number:
JP2001508352A
Publication Date:
February 04, 2003
Filing Date:
June 30, 2000
Export Citation:
Assignee:
GLAXO GROUP LIMITED
International Classes:
C12N15/09; C12N1/16; C12N1/19; C12N1/20; C12N1/21; C12N9/02; C12N9/10; C12N9/12; C12N9/78; C12N15/52; C12N15/53; C12N15/54; C12N15/55; C12P19/38; C12R1/19; C12R1/86; (IPC1-7): C12N15/09; C12N1/16; C12N1/19; C12N1/20; C12N1/21; C12P19/38
Attorney, Agent or Firm:
Kenji Yoshitake (4 others)