Title:
【発明の名称】細胞サブセットの欠落/陽性選択による免疫細胞の免疫療法活性の増強方法
Document Type and Number:
Japanese Patent JPH06506465
Kind Code:
A
Abstract:
The present invention provides a method for enhancing the immunotherapeutic activity, e.g., cytotoxicity, of immune cells by depleting immune cells of a cell subset that down-regulates the immune response, such as either CD4+ or CD8+ lymphocytes. The remaining depleted immune cells are then cultured in the presence of interleukin-2 (IL-2) and an antibody to a lymphocyte surface receptor, preferably an anti-CD3 monoclonal antibody (MoAb). The present invention also provides a method of enhancing the immunotherapeutic activity, e.g., cytotoxicity, of immune cells by culturing immune cells in the presence of IL-2 and an antibody to a lymphocyte surface receptor, preferably an anti-CD3 MoAb; separating a cell subset capable of developing immunotherapeutic activity, e.g., cytotoxicity, from the cultured immune cells, e.g., either CD4+ or CD8+ lymphocytes; and then subculturing the separated lymphocytes in the presence of IL-2.
Inventors:
Ocoa, Augusto Carlos
Geller, Robin Lee
Back, frit h
Geller, Robin Lee
Back, frit h
Application Number:
JP50938992A
Publication Date:
July 21, 1994
Filing Date:
April 06, 1992
Export Citation:
Assignee:
Regents of the University of Minnesota
International Classes:
A61K35/14; A61K35/17; A61K38/20; A61K39/395; C07K16/28; C12N5/00; C12N5/02; C12N5/0783; A61K9/127; A61K35/12; A61K39/00; (IPC1-7): A61K35/14; A61K39/395; C12N5/08
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)