Title:
DETECTION AND DETERMINATION OF SUPEROXIDE RADICAL
Document Type and Number:
Japanese Patent JP3477651
Kind Code:
B2
Abstract:
PURPOSE: To make it possible to measure superoxide radical with more higher sensitivity by making the superoxide radical react with pyrido-pyridazine derivative or the salt thereof, and performing chemical light emission.
CONSTITUTION: Superoxide-radical (02-) and pyride-pyridazine derivative or the salt thereof expressed by the formula are made to react, and chemical light emission is generated. In the formula, R1 indicates hydrocarbon or hetero- ring group, which can be substituted, respectively. R2 indicates hydrogen, hydroxyl group, thyol and the like R3 indicates hydrogen atoms, oxygen atoms and hydroxyl group, which can be substituted X indicates oxygen atoms or sulfur atoms. As a sample, which is applied in this method, it is preferable to apply the sample in the measurement of 02-, which is produced from, e.g. cells. As the cells producing 02-, there are the leukocyte-based, vascular-system-based and nervous-system-based cells and the like. When this method is used, the specific light emitting quantity and background light emitting quantity are high, 02- can be detected with high sensitivity and the quantity can be determined.
Inventors:
Yuzo Ichimori
Yumiko Nishinaka
Toru Sugawara
Yumiko Nishinaka
Toru Sugawara
Application Number:
JP697694A
Publication Date:
December 10, 2003
Filing Date:
January 26, 1994
Export Citation:
Assignee:
Takeda GmbH
International Classes:
G01N21/76; G01N33/50; G01N33/84; C07D471/04; (IPC1-7): G01N21/76; G01N33/84
Attorney, Agent or Firm:
Sekiguchi Yo (2 people outside)