光学的方法による膜内外電位差の検出 - The Regents of the University of California

Title:

光学的方法による膜内外電位差の検出

Document Type and Number:

Japanese Patent JP4033899

Kind Code:

B2

Abstract:

Methods and compositions are provided for determining the potential of a membrane. In one aspect, the method comprises: (a) introducing a first reagent comprising a hydrophobic fluorescent ion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane, as described by the Nernst equation, (b) introducing a second reagent which labels the first face or the second face of the membrane, which second reagent comprises a chromophore capable of undergoing energy transfer by either (i) donating excited state energy to the fluorescent ion, or (ii) accepting excited state energy from the fluorescent ion, (c) exposing the membrane to radiation; (d) measuring energy transfer between the fluorescent ion and the second reagent, and (e) relating the energy transfer to the membrane potential. Energy transfer is typically measured by fluorescence resonance energy transfer. In some embodiments the first and second reagents are bound together by a suitable linker. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes.

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Inventors:

Zien, Roger, Wye.
Gonzalez, Jesus, E., 3rd

Application Number:

JP50192697A

Publication Date:

January 16, 2008

Filing Date:

June 06, 1996

Export Citation:

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Assignee:

The Regents of the University of California

International Classes:

G01N21/78; G01N33/52; C07D209/08; C07D213/74; C07D215/22; C07D215/48; C07D215/50; C07D215/54; C07D239/60; C07D239/62; C07D239/66; C07D311/08; C07D311/16; C07D335/06; C07F5/00; C07F5/02; C07F9/10; C07F9/572; C09B23/02; C09K11/06; G01N21/76; G01N33/50; G01N33/542; G01N33/58; G01N33/68

Domestic Patent References:

JP7063756A
JP7103983A
JP5249605A
JP2217867A

Other References:

D.Witzak, ”Oxonol-Luminophore mit funktionalisierten Seitenkette” Diplomarbeit, 1994 Heinrich-Hein
S.Bechtel”Synthese von Styrylfarbstoffen mit Reaktivgruppen” Diolomarbeit, 1994 Heinrich-Heine U
BIOCHEMISTRY VOL.26,NO.14(1987) p.4279-4287
希土類第16号(1990)第116-117頁

Attorney, Agent or Firm:

Yusuke Hiraki
Ishii Teiji
Naomi Shimamura

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