Title:
光学的方法による膜内外電位差の検出
Document Type and Number:
Japanese Patent JP4064945
Kind Code:
B2
Abstract:
Methods and compositions are provided for determining the potential of a membrane. In one aspect, the method comprises: (a) introducing a first reagent comprising a hydrophobic fluorescent ion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane, as described by the Nernst equation, (b) introducing a second reagent which labels the first face or the second face of the membrane, which second reagent comprises a chromophore capable of undergoing energy transfer by either (i) donating excited state energy to the fluorescent ion, or (ii) accepting excited state energy from the fluorescent ion, (c) exposing the membrane to radiation; (d) measuring energy transfer between the fluorescent ion and the second reagent, and (e) relating the energy transfer to the membrane potential. Energy transfer is typically measured by fluorescence resonance energy transfer. In some embodiments the first and second reagents are bound together by a suitable linker. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes.
Inventors:
Zien, Roger, Wye.
Gonzalez, Jesus, E., 3rd
Gonzalez, Jesus, E., 3rd
Application Number:
JP2004131475A
Publication Date:
March 19, 2008
Filing Date:
April 27, 2004
Export Citation:
Assignee:
The Regents of the University of California
International Classes:
C07F5/02; G01N21/78; C07D209/08; C07D213/74; C07D215/22; C07D215/48; C07D215/50; C07D215/54; C07D239/60; C07D239/62; C07D239/66; C07D311/08; C07D311/16; C07D335/06; C07F5/00; C07F9/10; C07F9/572; C09B23/02; C09K11/06; G01N21/76; G01N33/50; G01N33/52; G01N33/542; G01N33/58; G01N33/68
Domestic Patent References:
| JP6247981A | ||||
| JP6247979A |
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Naomi Shimamura
Ishii Teiji
Naomi Shimamura