Title:
DETECTION METHOD OF RESISTANCE TO POTATO DISEASE AND PEST USING DNA MARKER LINKED WITH R3 GENE
Document Type and Number:
Japanese Patent JP2012055173
Kind Code:
A
Abstract:
To provide a detection method for efficiently growing resistant varieties by utilizing a detection method of resistance using DNA markers and allowing individuals resistant to potato disease and pest to be detected in DNA level and in a short period of time.
DNA markers are composed of primers for DNA marker detection linked with R3 genes expressed in the following specific sequence: a synthetic primer of 20 bases of "5'-TGCCAAATGACTTGTTTATC-3'" and a synthetic primer of 19 bases of "5'-CAAATATAACCTGTCGACG-3'"; or a synthetic primer of 20 bases of "5'-CTTCTCCTGTCATCTTGTTA-3'" and a synthetic primer of 19 bases of "5'-CAAATATAACCTGTCGACG-3'".
Inventors:
OBAYASHI KENGO
Application Number:
JP2010198527A
Publication Date:
March 22, 2012
Filing Date:
September 06, 2010
Export Citation:
Assignee:
NAGASAKI PREFECTURE
International Classes:
C12Q1/68; C12N15/09
Other References:
JPN6014049195; Ｍｏｌｅｃｕｌａｒ Ｐｌａｎｔ-Ｍｉｃｒｏｂｅ Ｉｎｔｅｒａｃｔｉｏｎｓ １７， ４, 2004, ４２８-４３５
JPN6014049197; Ｒｕｓｓｉａｎ Ａｇｒｉｃｕｌｔｕｒａｌ Ｓｃｉｅｎｃｅｓ ５, 201010, ３３４-３３７
JPN6014049194; Ｍｏｌｅｃｕｌａｒ Ｐｌａｎｔ-Ｍｉｃｒｏｂｅ Ｉｎｔｅｒａｃｔｉｏｎｓ ２２， １２, 2009, １５３５-１５４５
JPN7014003318; ＰＰＯ-ｓｐｅｃｉａｌ ｒｅｐｏｒｔ １４, 20100503, ２３１-２３８
JPN6014049191; Ｔｈｅ Ｐｌａｎｔ Ｊｏｕｒｎａｌ ４２, 2005, ２５１-２６１
JPN7015001739; 長崎県農林技術開発センター研究報告 １号, 201003, １-２５
JPN6014049197; Ｒｕｓｓｉａｎ Ａｇｒｉｃｕｌｔｕｒａｌ Ｓｃｉｅｎｃｅｓ ５, 201010, ３３４-３３７
JPN6014049194; Ｍｏｌｅｃｕｌａｒ Ｐｌａｎｔ-Ｍｉｃｒｏｂｅ Ｉｎｔｅｒａｃｔｉｏｎｓ ２２， １２, 2009, １５３５-１５４５
JPN7014003318; ＰＰＯ-ｓｐｅｃｉａｌ ｒｅｐｏｒｔ １４, 20100503, ２３１-２３８
JPN6014049191; Ｔｈｅ Ｐｌａｎｔ Ｊｏｕｒｎａｌ ４２, 2005, ２５１-２６１
JPN7015001739; 長崎県農林技術開発センター研究報告 １号, 201003, １-２５