Title:
DETECTION OF TARGET SUBSTANCE IN SPECIMEN
Document Type and Number:
Japanese Patent JP3796271
Kind Code:
B2
Abstract:
PURPOSE: To detect a target substance in high sensitivity without causing B/F separation by reacting a specimen with plural reagents containing pyrylium(- analog) compound constituting a reaction system varying by a mutual action interposing the target substance and determining the change of the reaction system.
CONSTITUTION: A target substance in a specimen is detected in high sensitivity without causing B/F separation by reacting a specimen with two kinds of reagents containing a pyrylium compound, a pyrylium-analog compound, etc. constituting a reaction system varying by a mutual action interposing the target substance and expressed by formula I {the ring is a heterocycllc group expressed by formula II (X is 0, S2 Se or Te), pyrylium ring or pyrylium-analog ring; R1 and R2 each is H, a halogen, a sulfonate, amino, styryl, nitro, OH, COOH, cyano, a (substituted)alkyl, a (substituted)aryl, etc.; R3 is A [A is a (substituted) aryl or CH=R5 (R5 is a heterocyclic group, a cycloalkyl, an aromatic ring, etc.)] or L-A (L is CH=CH, an arylene, an ankylene, etc.)} and determining the change of the reaction system.
Inventors:
Nobuko Yamamoto
Takashi Okamoto
Takashi Okamoto
Application Number:
JP11262694A
Publication Date:
July 12, 2006
Filing Date:
May 26, 1994
Export Citation:
Assignee:
Canon Inc
International Classes:
C12Q1/68; G01N33/58; C07D309/34; C07D335/02; C07D409/04; C12N15/09; G01N33/53; (IPC1-7): C12Q1/68; G01N33/58; //C07D309/34; C07D335/02; C07D409/04; C12N15/09
Domestic Patent References:
| JP62240864A | ||||
| JP6066802A | ||||
| JP6153997A |
Other References:
Nucleic Acids Symposium Series,No.29, p.83-84
Cytometry,1984, Vol.5, p.339-347
J.Am.Chem.Soc., 1992, Vol.114, pp.3656-3660
Photochemistry and Photobiology, 1991, Vol.53, No.4, pp.555-558
The FASEB Journal, 1988, Vol.2, No.5, 4256
Cytometry,1984, Vol.5, p.339-347
J.Am.Chem.Soc., 1992, Vol.114, pp.3656-3660
Photochemistry and Photobiology, 1991, Vol.53, No.4, pp.555-558
The FASEB Journal, 1988, Vol.2, No.5, 4256
Attorney, Agent or Firm:
Ohta Kengaku
Akio Miyazaki
Ishibashi Masayuki
Shinichi Iwata
Masaaki Ogata
Akio Miyazaki
Ishibashi Masayuki
Shinichi Iwata
Masaaki Ogata