To provide a method and device for purifying a molecule of interest from a heterogeneous clarified fluid mixture.
The device generally includes a continuous perfusion fermentation system, a continuous particle removal system integrated with the perfusion fermentation system; and a continuous purification system integrated with the particle removal system, which is maintained under sterile conditions. The method includes filtering the heterogeneous clarified fluid mixture by continuous ultrafiltration at a specific flow rate below the transition point of the molecule of interest in the pressure-dependent region of the flux versus TMP curve, wherein the specific flow rate is maintained substantially constant throughout the continuous ultrafiltration.
GIOVANNINI ROBERTO
KONSTANTINOV KONSTANTIN B
NGUYEN HUONG MY
WU PENG
| JPH06500730A | 1994-01-27 | |||
| JPH06500730A | 1994-01-27 |
| WO2004076695A1 | 2004-09-10 | |||
| US6544424B1 | 2003-04-08 | |||
| WO2003020919A2 | 2003-03-13 | |||
| WO2004076695A1 | 2004-09-10 | |||
| US6544424B1 | 2003-04-08 |
JPN6014006362; Ｂｉｏｔｅｃｈｎｏｌ． Ｂｉｏｅｎｇ． ｖｏｌ．８２， ｎｏ．７, 2003, ｐｐ．７５１-７６５
JPN6014006364; Ｐｈａｒｍ． Ｔｅｃｈｎｏｌ． ｖｏｌ．２７， ｎｏ．３, 2003, ｐｐ．６２-７６
JPN6014006358; Ｃｙｔｏｔｅｃｈｎｏｌｏｇｙ ｖｏｌ．２８， ｎｏ．１-３, 1998, ｐｐ．１６３-１７５
JPN6014006362; Ｂｉｏｔｅｃｈｎｏｌ． Ｂｉｏｅｎｇ． ｖｏｌ．８２， ｎｏ．７, 2003, ｐｐ．７５１-７６５
JPN6014006364; Ｐｈａｒｍ． Ｔｅｃｈｎｏｌ． ｖｏｌ．２７， ｎｏ．３, 2003, ｐｐ．６２-７６
Hajime Asamura
Yukihiro Ikeda
Naganuma Akio