Title:
内部標準の存在下で色素を標識することによる閉じた膜構造上の細胞表面タンパク質の示差分析
Document Type and Number:
Japanese Patent JP2006526137
Kind Code:
A
Abstract:
Disclosed are matched fluorescent reagents and a method for reproducibly labelling membrane components, such as those expressed on the cell surface, and subsequent differential analysis of the labelled components to detect differences between cell types and states. Furthermore, the present method utilises an internal standard in order to match protein patterns across gels thereby avoiding gel-to-gel variation. The method according to the invention is particularly useful, for example, for detecting low abundance membrane proteins, for detecting changes in receptors expressed in the cell membrane, for example on ligand binding, or in response to stimuli.
Inventors:
Cops, Catherine
Fowler, Susan Janet
Holy, Imogen
Sweet, Alison Claire
Fowler, Susan Janet
Holy, Imogen
Sweet, Alison Claire
Application Number:
JP2005500161A
Publication Date:
November 16, 2006
Filing Date:
May 28, 2003
Export Citation:
Assignee:
GE Healthcare UK Limited
International Classes:
G01N33/48; C07K1/13; G01N21/76; G01N27/447; G01N30/74; G01N30/88; G01N33/50; G01N33/569; G01N33/58; G01N33/68; C09B23/00
Domestic Patent References:
| JPH02191674A | 1990-07-27 | |||
| JPH1088012A | 1998-04-07 | |||
| JPH01199949A | 1989-08-11 | |||
| JP2002207041A | 2002-07-26 | |||
| JP2000350599A | 2000-12-19 | |||
| JP2003506718A | 2003-02-18 |
Attorney, Agent or Firm:
Kenichi Matsumoto
Hirokazu Ogura
Toshihisa Kurokawa
Hirokazu Ogura
Toshihisa Kurokawa