DIGOXIGENIN DERIVATIVE CONTAINING CARBODIIMIDE GROUP

Japanese Patent JP3798064

B2

PROBLEM TO BE SOLVED: To obtain the subject new derivative consisting of a specific digoxigenin derivative containing carbodiimide group, useful as a determination label for nucleic acid detection method or immunoassay to enable highly sensitive analysis by a simple labeling operation and enabling the labeling of a natural nucleic acid.
SOLUTION: This new digoxigenin derivative containing carbodiimide group is expressed by formula I {Z is a digoxigenin-containing group expressed by formula II [A is O, COO or NHCOO; L is an alkylene which may contain a group such as NHCO, CONH, O, S, NR (R is an alkyl), COO or OCO in the main chain; W is quaternary ammonium group; Y1 and Y2 are each a straight- chain bond or same as L; B is H or a univalent organic group same as or different from a group of formula W-Z}. The derivative is useful as a labeling substance in nucleic acid detection method, immunoassay, etc. The compound can be produced by reacting a carbodiimide compound of formula III [W' is a (substituted) amino group] with a halogenated digoxigenin derivative expressed by Z-X (X is a halogen).

Suzuki Osamu
Masuda Gen
Namiko Shiohata
Kazuko Matsumoto

JP11392296A

July 19, 2006

May 08, 1996

Click for automatic bibliography generation  

Nisshinbo Corporation

C07J19/00; G01N33/532; C07J41/00; C07J43/00; G01N33/533; G01N33/58; (IPC1-7): C07J19/00; G01N33/532

US4895955

Molecular and Cellular Probes,1991, Vol.5, No.3,pp.161-205
Nucleic Acid Symposium Series,1995, Vol.34,pp.69-70

Tsutomu Toyama
Hidemi Matsukura
Yoshiyuki Kawaguchi

Previous Patent: ANTENNA WITH CHANGE-OVER MECHANISM

Next Patent: FAN MOTOR COOLING APPARATUS AND BURNER PROVIDED WITH THE FAN MOTOR COOLING APPARATUS