Title:
ANTIBODY TO AMINO-TERMINAL PROPEPTIDE OF TYPE I PROCOLLAGEN AND ASSAY USING THE SAME
Document Type and Number:
Japanese Patent JP3245052
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a new amino terminal propepticle that comprises an intact trimer of procollagen type I isolated without a proteolytic enzyme and is useful as an immunogen for preparation of an antibody for immunoassay of this N- terminal-propeptide.
SOLUTION: This is a mew α1-homotrimer type amino terminal propeptide that is an intact trimer of the procollagen of type I isolated with no use of proteolytic enzyme. This is useful as an immunogen for producing an antibody to be used in the immunoassay of this amino terminal propeptide and permits the determination of the propeptide of the type I procollagen without the inconvenience that is accompanied by simultaneous determination of its monomer form. This propeptide is obtained by saturating the raw material containing this propeptide by ≤50% saturation with ammonium sulfate, subjecting the precipitate to anionic ion exchange chromatography at a pH of 6.5-7.5, eluting the fractions by the concentration gradient of sodium chloride and subjecting the active fractions to the reverse phase separation at a pH of 6.5-7.5.
Inventors:
Yuharisteri
Leila Listeri
Yuka Melco
Cyla Kaupira
Leila Listeri
Yuka Melco
Cyla Kaupira
Application Number:
JP12246896A
Publication Date:
January 07, 2002
Filing Date:
April 18, 1996
Export Citation:
Assignee:
Orion-Yuhichime Salmon Yukiture
International Classes:
C07K1/20; C07K14/78; C07K16/00; C07K16/18; G01N33/68; A61K39/00; (IPC1-7): C07K14/78; C07K16/18; G01N33/68
Domestic Patent References:
| JP1139600A |
Other References:
Ｃｌｉｎｉｃａｌ Ｃｈｅｍｉｓｔｒｙ，（１９９３）Ｖｏｌ．３９，Ｎｏ．１１，ｐ．２２５４−２２５８
Ｊｏｕｒｎａｌ ｏｆ Ｂｏｎｅ ａｎｄ Ｍｉｎｅｒａｌ Ｒｅｓｅａｒｃｈ，（１９９２）Ｖｏｌ．７，Ｎｏ．１１，ｐ．１２４３−１２５０
Ｊｏｕｒｎａｌ ｏｆ Ｂｏｎｅ ａｎｄ Ｍｉｎｅｒａｌ Ｒｅｓｅａｒｃｈ，（１９９２）Ｖｏｌ．７，Ｎｏ．１１，ｐ．１２４３−１２５０
Attorney, Agent or Firm:
Sota Asahina (2 outside)