PURPOSE: To efficiently produce tryptophanase by culturing an organic cell, etc., transformed with a recombinant plasmid containing a DNA sequence capable of coding the tryptophanase derived from a strain of the genus Proteus.
CONSTITUTION: A DNA fragment mixture containing a gene capable of coding tryptophanase derived from a strain of the genus Proteus is linked with a vector DNA, such as plasmid vector DNA, to prepare a recombinant plasmid, which is subsequently introduced into an organic cell or microorganism to afford a transformant having a desired genetic character and a character of the vector DNA. The resultant transformant is then subjected to shaking culture with aeration, etc., in an LB culture medium, etc., to grow and accumulate the tryptophanase in the microbial cells. The recovered tryptophanase is subsequently purified by ultrasonic crushing, mechanical crushing using glass beads, etc.
| WO/1999/006566 | PRODUCTION OF TRANSGENIC POINSETTIA |
| WO/2013/055667 | BIOCATALYSIS CELLS AND METHODS |
| JP2024511096 | Pharmaceutical combinations to treat cancer |
SOGABE YUKIHIRO
OKA MASANORI
