To obtain the subject new DNA having a specific base sequence, containing a domain to conduct the transcription control of a human high- affinity IgE receptor α-strand gene, and capable of efficiently producing a high- affinity IgE receptor to start I-type allergic reaction.
This new DNA contains a base sequence of the formula and also contains a domain to conduct the transcription control of a human high- affinity IgE receptor(Fc RI) α-strand gene. This DNA is expressed on the cell membrane of a mast cell or basophilic cell, being capable of efficiently producing a high-affinity IgE receptor(Fc RI) to start I-type allergic reaction along with being capable of controlling, as a promoter, the expression of a foreign protein gene. This DNA is obtained by conducting a PCR of a human chromosome DNA as template purified from human peripheral blood by the use, as primer, of e.g. a synthetic oligonucleotide prepared from the 5' terminal side base sequence of a human high-affinity IgE receptor cDNA.
RA TOMOYASU
OKUMURA YASUSHI
TORII YAKUHIN KK
NIKKA WHISKY
RA TOMOYASU